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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Topic 1: Urinalysis and Other Body Fluids5-10%- Urine formation and composition
- Physical, chemical, and microscopic analysis
- Semen and amniotic fluid testing
- Cerebrospinal, serous, and synovial fluids
Topic 2: Immunology and Serology5-10%- Immunologic testing methodologies
- Immune system principles and components
- Autoimmune and immunodeficiency disorders
- Infectious disease serology
Topic 3: Laboratory Operations5-10%- Laboratory mathematics and instrumentation
- Pre-analytical, analytical, and post-analytical processes
- Laboratory safety and regulatory compliance
- Quality control and quality assurance
Topic 4: Hematology20-25%- Red blood cell physiology and disorders
- Routine and automated hematology procedures
- White blood cell morphology and diseases
- Hemostasis and coagulation testing
Topic 5: Clinical Chemistry20-25%- Carbohydrates, lipids, proteins, and enzymes
- Electrolytes, acid-base balance, and blood gases
- Toxicology and liver/kidney function tests
- Endocrinology and therapeutic drug monitoring
Topic 6: Microbiology15-20%- Bacteriology and antimicrobial susceptibility
- Identification of pathogenic microorganisms
- Mycology, parasitology, and virology
- Specimen collection and culture techniques
Topic 7: Blood Banking (Immunohematology)15-20%- Transfusion reactions and adverse events
- Antibody detection and identification
- Blood group systems and antigens
- Compatibility testing
- Blood components and storage

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. RAST tests, or Radioallergosorbent tests, are used to screen for an allergy to a specific substance or substances if a person presents with allergy-like symptoms.
The assay which is most helpful in identifying specific allergens is:

A) RIA
B) Complement fixation
C) RAST
D) C-reactive proteins


2. Autologous units must be drawn before they are needed, and must be readily available, therefore are generally not of use in emergencies.
All of the following are benefits of autologous donation except:

A) Eliminates sensitization to cellular blood components
B) Reduces exposure to infectious agents
C) Are always on hand in case of an unexpected emergency
D) Reduces demand for homologous blood


3. Epidemiology studies only infectious diseases that impact humans

A) True
B) False


4. The clue to the identification of the colony seen in the upper frame is the gram stain in the lower frame, in which are seen short, rounded, gram positive bacilli, many of which possess distinct spores. As the colonies grew aerobically, the presence of spores indicates Bacillus species. The colonies are spreading, smooth, yellow-white and non-hemolytic. The lack of hemolysis and the small size of the bacterial cells suggests a species other than Bacillus cereus, the species causing most human infections. The isolate was identified as Bacillus circulans, which is consistent with the gram stain morphology. Clostridium septicum also produces spores; however, this species is an anaerobe and would grow poorly if at all and not produce spores aerobically. Listeria monocytogenes and Lactobacillus species are gram positive bacilli; however, neither of these produce spores.
The colonies seen in the upper frame, grown after 48 hours incubation aerobically at 35°C, and the accompanying gram stain in the lower frame are uncommonly associated with human disease, but have been associated with septicemia, bronchopneumonia, osteomyelitis and other infections, particularly in intravenous drug users. The most likely identification is:

A) Lactobacillus species
B) Listeria monocytogenes
C) Clostridium septicum
D) Bacillus circulans


5. The beta hemoglobin chain loci are found on which chromosome?

A) Chromosome 16
B) Chromosome 11


Solutions:

Question # 1
Answer: C
Question # 2
Answer: C
Question # 3
Answer: A
Question # 4
Answer: D
Question # 5
Answer: B

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